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high-throughput screening (HTS) laboratory, which is located
at the Hauptman-Woodward Institute (HWI), will prepare crystal-growth
screening experiments in 1536-well microassay plates for a nominal
fee. (This fee
will be waived for academic users requesting the specialized membrane
protein crystallization screen.*)
Information will not be publicly disclosed regarding any sample received for crystallization trials without the expressed written consent of the investigator(s). However, investigators will be asked to give certain information about the target protein for inclusion in the crystallization database that is being compiled as part of the ongoing research program in crystal growth at HWI. (Participation is voluntary, but is strongly encouraged and deeply appreciated.) The database is composed largely of results from experiments conducted in the high-throughput laboratory, and it will be used to predict crystallization recipes for proteins that have never been crystallized successfully. The prediction algorithm depends on the identification of proteins that have been crystallized and are similar to the problem at hand. Measures of similarity based on biochemical and biophysical properties are being devised.
If the high-throughput screening facility assists you in identifying initial crystallization conditions, then to help us track the use and success of the screening laboratory, we ask you to please reference one of the following articles:
Standard Screen. Luft, J. R., Collins, R. J., Fehrman, N. A., Lauricella, A. M., Veatch, C. K. & DeTitta, G. T. (2003). A deliberate approach to screening for initial crystallization conditions of biological macromolecules. J. Struct. Biol. 142, 170-179. [PubMed ID: 12718929]
Membrane Screen. Koszelak-Rosenblum, M., Krol, A., Mozumdar, N., Wunsch, K., Ferin, A., Cook, E., Veatch, C. K., Nagel, R., Luft, J. R., DeTitta, G. T & Malkowski, M. G. (2009). Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins. Protein Science 18, 1828-1839. [Pub Med ID: 19554626]
* Industrial users should contact Mike Malkowski (716-898-8624) for pricing information regarding the membrane screen.