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Dr. Mark Dumont’s laboratories are located in the Center for Pediatric Biomedical Research in the MRBX building of the University of Rochester Medical Center, and they are affiliated with the Department of Pediatrics.
Dr. Dumont has extensive experience in the fields of yeast biology and biochemistry, and he has been involved in several genomic-scale projects to clone, express and purify proteins from E. coli as well as from the yeast Saccharomyces cerevisiae. He is now focusing on the development of technology for the use of S. cerevisiae for the expression of proteins for crystal structural analysis, with emphasis on high-throughput production of yeast membrane proteins. Use of a eukaryotic organism such as yeast for expression of proteins is desirable because of the lack of solubility of a large fraction of eukaryotic proteins that are examined in E. coli, because many of the natural post-translational modifications are not found in E. coli but are found in yeast, and because yeast is simple and easy to grow and transform. Furthermore, yeast can be easily engineered to express modification enzymes as needed for the proper folding of a eukaryotic target protein, to remove unwanted proteases, and to introduce other desirable properties. [Learn more]